FangShengResearch Accociate, UCSD

Skaggs School of Pharmacy and Pharmaceutical Sciences

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I obtained my PhD in Structural Biology at Michigan State University in 2008. Currently I am protein purification specialist working for Drs. Podust and McKerrow laboratories. I am also responsible for managing the high-throughput crystallization facility – the Crystal Lab.

On the research side, I focus on the structure determination of drug-protein target complexes to assist structure-based rational drug design. Under Dr. Podust guidance, I purify and characterize enzymes and their mutants to illustrate the mechanism of oxidative enzymatic reactions in bacterial biosynthesis systems producing antibiotics.

On the service side, I maintain and manage our modern crystallization facility. I provide initial training and unreserved expertise to help researchers crystallize the biological macromolecules of their interest. Please do not hesitate to contact me if you are interested using the service or have any questions regarding our facility. My source of enjoyment is crystals.

PUBLICATIONS

Biochemical and Structural Characterization of MycCI, a Versatile P450 Biocatalyst from the Mycinamicin Biosynthetic Pathway. ACS Chem Biol. 2016 Aug 5. [Epub ahead of print] DeMars MD 2nd, Sheng F, Park SR, Lowell AN, Podust LM, Sherman DH.

X-ray structures of thioredoxin and thioredoxin reductase from Entamoeba histolytica and prevailing hypothesis of the mechanism of Auranofin action. J Struct Biol. 2016 May;194(2):180-90. doi: 10.1016/j.jsb.2016.02.015. Epub 2016 Feb 12. Parsonage D, Sheng F, Hirata K, Debnath A, McKerrow JH, Reed SL, Abagyan R, Poole LB, Podust LM.

Oligosaccharide binding in Escherichia coli glycogen synthase. Biochemistry. 2009 Oct 27;48(42):10089-97. doi: 10.1021/bi900916t. Sheng F, Yep A, Feng L, Preiss J, Geiger JH.

The crystal structures of the open and catalytically competent closed conformation of Escherichia coli glycogen synthase. J Biol Chem. 2009 Jun 26;284(26):17796-807. doi: 10.1074/jbc.M809804200. Epub 2009 Feb 25. Sheng F, Jia X, Yep A, Preiss J, Geiger JH.